Research Chemist in Molecular Biology, Danith Ly Group
Carnegie Mellon University
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Research Chemist in Molecular Biology, Danith Ly Group

Carnegie Mellon University

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Details
Posted:
January 30, 2021
Location:
Pittsburgh, PA
Salary:
Open

Carnegie Mellon is seeking an experienced molecular biologist with expertise in biochemistry and molecular biology, immunology, and microbiology techniques; cell and tissue culture, animal handling, and fluorescent imaging.

DUTIES & RESPONSIBILITIES

  • Employ the latest recombinant DNA techniques to engineer cells and animal models.
    Recombinant virus construction, SNP typing, gene cloning, transfection, transduction, PCR, DNA & RNA isolation, cDNA preparation, qPCR and DNA sequencing, fluorescence spectrophotometer, electrophoresis, Western blotting, Crispr-Cas9, immunoprecipitation (IP), and co-IP.
  • Application of multicolor flow cytometry, ELISA, bioplex, fluorescence microscopy, confocal microscopy, in vivo imaging and image stream to address molecular biology and immunological problems.
  • Cell and tissue culture, PBMC isolation, primary B-cell isolation and culture, stable cell line establishment.
  • Virus production, Mycobacterium culture, drug screening assays.
  • Animal tumor models (melanoma, colon and renal), ip injection, iv injection, and sc injection, tail blood withdrawal, survival surgery and tumor development in SCID mice transfection, and stable cell preparation.
  • Primer designing, Corel Draw, Graph Pad Prism, Cell Quest, FlowJo, End Note, Zeiss LSM browser, Image J, and Quantity one, basic bioinformatics tools.
  • Design and execute experiments, data collection, preparation of manuscripts, and presentations.
  • Preparation of grant proposals and budget management.

EDUCATION

  • PhD in Molecular Biology and/or Immunology.

EXPERIENCE

  • Ph.D. degree with 5+ years of research experience in academia and/or industry.
  • A proven track record of accomplishment in the research field: publications, patents, conference presentations.
  • A strong track record of working in collaboration with partners from different organizations and scientific expertise.

TECHNICAL KNOWLEDGE

  • Experienced in the following areas is highly desirable: animal handling, microbiology, cell and tissue culture, immunology, fluorescence imaging, biochemistry, molecular biology, and bioinformatics.

QUALITIES

  • Committed to achieve the highest standards of quality work.
  • Decision making and quickly assessing available information.
  • Commitment in delivering accurate results of the project in the defined timeframe.
  • The ability to plan and prioritize workload and to be able to perform under high pressure.
  • The ability to build and maintain contacts with team members and responding positively to changing circumstances.
  • Demonstrated skill both writing and presenting scientific reports.

Interested candidate applications consisting of a cover letter outlining their skill set and a detailed CV should be submitted through Interfolio.

The evaluation of candidates will begin immediately and continue until the available position is filled.

Carnegie Mellon University is an equal opportunity employer and is committed to increasing the diversity of its community on a range of intellectual and cultural dimensions.  Carnegie Mellon welcomes applicants who will contribute to this diversity through their research, teaching, and service, including women, members of minority groups, protected veterans, individuals with disabilities, and others who would contribute in different ways.

Carnegie Mellon University shall abide by the requirements of 41 CFR §§ 60-1.4(a), 60-300.5(a) and 60-741.5(a). These regulations prohibit discrimination against qualified individuals based on their status as protected veterans or individuals with disabilities, and prohibit discrimination against all individuals based on their race, color, religion, sex, or national origin. Moreover, these regulations require that covered prime contractors and subcontractors take affirmative action to employ and advance in employment individuals without regard to race, color, religion, sex, national origin, protected veteran status or disability.

Internal Number: 74703
About Carnegie Mellon University
Carnegie Mellon (www.cmu.edu) is a private, internationally ranked research university with programs in areas ranging from science, technology and business, to public policy, the humanities and the arts. More than 12,000 students in the university’s seven schools and colleges benefit from a small student-to-faculty ratio and an education characterized by its focus on creating and implementing solutions for real problems, interdisciplinary collaboration and innovation. A global university, Carnegie Mellon’s main campus in the United States is in Pittsburgh, Pa. It has campuses in California’s Silicon Valley and Qatar, and programs in Africa, Asia, Australia, Europe and Mexico.
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