Carnegie Mellon is seeking an experienced molecular biologist with expertise in biochemistry and molecular biology, immunology, and microbiology techniques; cell and tissue culture, animal handling, and fluorescent imaging.
DUTIES & RESPONSIBILITIES
Employ the latest recombinant DNA techniques to engineer cells and animal models. Recombinant virus construction, SNP typing, gene cloning, transfection, transduction, PCR, DNA & RNA isolation, cDNA preparation, qPCR and DNA sequencing, fluorescence spectrophotometer, electrophoresis, Western blotting, Crispr-Cas9, immunoprecipitation (IP), and co-IP.
Application of multicolor flow cytometry, ELISA, bioplex, fluorescence microscopy, confocal microscopy, in vivo imaging and image stream to address molecular biology and immunological problems.
Cell and tissue culture, PBMC isolation, primary B-cell isolation and culture, stable cell line establishment.
Virus production, Mycobacterium culture, drug screening assays.
Animal tumor models (melanoma, colon and renal), ip injection, iv injection, and sc injection, tail blood withdrawal, survival surgery and tumor development in SCID mice transfection, and stable cell preparation.
Primer designing, Corel Draw, Graph Pad Prism, Cell Quest, FlowJo, End Note, Zeiss LSM browser, Image J, and Quantity one, basic bioinformatics tools.
Design and execute experiments, data collection, preparation of manuscripts, and presentations.
Preparation of grant proposals and budget management.
About Carnegie Mellon University
Carnegie Mellon (www.cmu.edu) is a private, internationally ranked research university with programs in areas ranging from science, technology and business, to public policy, the humanities and the arts. More than 12,000 students in the university’s seven schools and colleges benefit from a small student-to-faculty ratio and an education characterized by its focus on creating and implementing solutions for real problems, interdisciplinary collaboration and innovation. A global university, Carnegie Mellon’s main campus in the United States is in Pittsburgh, Pa. It has campuses in California’s Silicon Valley and Qatar, and programs in Africa, Asia, Australia, Europe and Mexico.